RNAi High-throughput screening

RNAi High-throughput screening

We deliver optimized candidate compounds precisely targeting your gene of interest, accompanied by comprehensive experimental reports.
All data are generated in accordance with MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) guidelines, ensuring high reliability and transparency.
Our high-throughput RNAi drug screening service is fully customized to meet your research and development objectives.

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Automated nucleic acid extraction system

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Real-time quantitative thermal block for 384 or 96 well RT-qPCR

Overview
siRNAgen Therapeutics offers a comprehensive, end-to-end RNAi drug development service, covering candidate sequence design, synthesis, and both in vitro and in vivo efficacy evaluation.
All data are generated in strict compliance with MIQE guidelines to ensure reliability and transparency, while automated high-throughput screening systems enable efficient and large-scale sample processing.
Leveraging extensive expertise in siRNA drug development, siRNAgen Therapeutics delivers customized candidate selection and provides clients with detailed experimental reports.
Key Strengths

1. Reliable results with MIQE guidelines

The MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) guidelines establish the essential standards and information required for the accurate and reliable reporting of quantitative real-time PCR experiments.
All experimental procedures and data analyses in this RNAi High-throughput screening service are conducted in strict accordance with these guidelines.
By adhering to internationally recognized MIQE standards, the objectivity and reliability of the results are greatly enhanced, ensuring that the generated data are suitable for scientific publication.

2. High-throughput screening

High-throughput screening is conducted using the ExiPrep™ 96 Lite automated nucleic acid extraction system and the Exicycler™ 384 real-time quantitative thermal block for 384-well RT-qPCR.
The integration of these automated systems ensures high operational efficiency and supports large-scale sample processing.

3. End-to-End solution

By conducting synthesis as well as both in vitro and in vivo efficacy evaluations in one place, you can significantly reduce both time and costs.

4. Expertise in siRNA Drug Development

We possess extensive know-how in siRNA drug development, ensuring reliable and effective research outcomes.

Analysis Result

Conventional algorithm-based designs may overlook certain highly potent drug candidates during the selection process.
Our service overcomes this limitation by sliding 1 nucleotide at a time across the target gene to generate all possible candidates, then thoroughly analyzing off-target effects and sequence similarity with model organisms to select as many candidates as possible.
This approach enables us to identify all highly effective candidates that may be overlooked by traditional methods.

CDMO Figure1. An example of identifying target gene drug candidates using a 1-nt sliding window algorithm.

Testing is conducted in accordance with the MIQE guidelines and utilizes automated equipment, enabling the rapid acquisition of highly reliable data.

CDMO Figure2. Validation of selected primer sets. (Shown for human ACTB gene)
CDMO Figure3. Results of high-throughput screening of 448 SAMiRNA candidates targeting Gene X
CDMO Figure4. Results of IC50 assay of SAMiRNA candidates targeting Gene X.

Our Pro-inflammatory Cytokine Response Test is a reliable method for verifying the induction of inflammatory immune responses and immunotoxicity.
The test analyzes changes in pro-inflammatory cytokine expression using RT-qPCR after treating human PBMCs with candidate compound.
This service allows you to scientifically demonstrate the safety of your candidates during the drug development stage.c.

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Figure5. Results of pro-inflammatory cytokine response test in human PBMCs using RT-qPCR

Our cytotoxicity tests provide an assessment of the safety of drug candidates using WST-1 and MTT assays.
Testing is performed using the standard MRC-5 cell line along with a diverse range of human-derived cell lines.
This approach allows us to provide toxicity results that are precisely tailored to your unique research goals.

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Figure6. Cytotoxicity test results with WST-1 assay in MRC-5 cells.

Service Details
Name Content
Design & synthesis Naked/Modified siRNA: On sequences between human and monkey by default. You can add animals such as mouse, rat, etc.
High-throughput screening
(RT-qPCR)
Screening experiment setup
Obtain suitable primers and cell lines for the experiment.
1st round screening: 
Screening all drug candidates
2nd round screening: 
Screening the top candidates for selection
3rd round screening: 
Top 5 candidates for IC50 testing
Other
  • Cytotoxicity assay: MTT or WST1
  • Innate immunity assay: Evaluate cytokine levels after treatment with human PBMCs by RT-qPCR
  • Protein Knockdown: Western blot or ELISA

* All assays are performed in triplicate for both biological and technical replicates.

Order

If you contact us(orders@sirnagen.com), we will promptly provide you with information on the experiment design and a quotation.
If you are interested in utilizing our SAMiRNA™ platform, please contact us for further information.